If creating v2 sample sheets for instruments in addition to those detailed, ensure the final sample sheet has the i5 index in the correct orientation as explained previously mentioned. Refer to Index 2 (i5) Orientation To find out more regarding how to enter i5 bases on the sample sheet in https://illuminatireal92456.liberty-blog.com/36843103/the-smart-trick-of-copyright-copyright-the-that-no-one-is-discussing